单片机汇编语言的教学研究

从单片机汇编语言实际教学的角度出发,在培养学生学习兴趣、合理选择教材、提高教师素质、优化教学方法等方面作了教学改革探讨,结合MCS一51单片机汇编语言教学中的应用,提出了一些具体建议和行之有效的方法,指出教师应针对不同的学生群体,注意多摸索一些实用的方法来实施教学。     

广西天堂山自然保护区两栖爬行动物资源调查

2011及2012年的6~8月,对广西天堂山自然保护区的两栖和爬行动物资源进行了调查研究。结果表明,天堂山保护区分布有两栖爬行动物共82种。其中有两栖类动物25种,隶属3目7科16属;爬行动物57种,隶属3目12科38属。根据其资源特点,提出了对天堂山自然保护区两栖爬行动物进行保护的对策与建议。     

天然橡胶初加工产品质量问题及控制方法

根据西双版纳勐捧制胶厂产品检验的结果,分析了影响天然橡胶初加工产品质量的因数,提出了控制天然橡胶初加工产品质量的方法。     

水稻主要抗瘟基因对福建稻瘟菌群体的抗性分析

用1995～2003年间在福建省水稻产区采集的稻瘟菌代表菌系的108个分离菌,它们在CO39近等基因系上测定被划分为30个毒性类型,用它们在30个水稻抗稻瘟病近等基因系或单基因系品种上进行抗病性测定.结果表明水稻抗稻瘟病基因Pi-kh抗性最强,抗性频率高达98.15%,Pi-1和Pi-9(t)也具有较高的抗性频率,是较好的抗源;对2个和3个Pi基因的联合抗性频率的分析,发现一些联合抗性频率极高,甚至有达到100%的组合,表明抗瘟育种采用多个Pi基因聚合,易于获得抗性强的品种.根据抗病基因与供试菌株互作的亲和性,对供试30个Pi基因可能的系统关系分析得到的初步信息可为抗病基因的聚合与布局策略提供参考.     

表达猪生长激素基因的重组腺病毒的构建及对猪生长的促进效果观察

利用复制缺陷型重组腺病毒作为基因表达载体,将猪生长激素（pGH）基因直接转导到猪体内细胞,研究猪生长激素基因在猪体内的促生长作用.用同源重组的方法,构建含pGH基因的重组腺病毒（Ad-pGH）.将其感染293细胞和PK-15细胞,均可检测到pGH.用1 mL此重组腺病毒（1010TCID50）一次性肌肉注射60日龄的杂交长白猪.结果表明,试验组的猪平均增重比对照组的猪增加37%（注射后第4周）和19%（注射后第6周）;饲料报酬提高28%（注射后第4周）和18%（注射后第6周）.试验结果证明,由重组腺病毒表达的pGH具有生物学活性,在猪体内起到明显的促进生长的作用.重组腺病毒介导的pGH基因在猪体内的表达可维持约4周.     

纳米技术在生物医学中的研究进展

20世纪80年代开始研究的纳米技术在90年代获得了突破性进展，它给许多行业带来巨大变化，它对生物医学的渗透与影响是显而易见的。利用纳米技术可将生物降解性和生物相容性的聚合物与药物一起制成纳米药物，作为靶向药物制剂，直接导入病灶部位的器官、组织甚至细胞，达到提高药物疗效，降低毒性的作用；将纳米材料作为药物载体，可增加某些药物的胃肠吸收，提高其生物利用度；将纳米材料作为载体，可用于基因的输送和治疗。文章就纳米技术在生物医学中的研究进展做一综述。     

猪臭鼻克雷伯氏菌的分离与鉴定

从呼吸系统症状明显、高热、耳朵等薄皮处发绀的病猪血与病死猪的心血、肝、脾中分离到1株细菌，经形态学观察、生化试验鉴定为臭鼻克雷伯氏菌；经人工感染小白鼠试验，表明具有较强的毒性。选用药敏试验筛选的敏感药物——头孢曲松钠治愈了病猪，控制了疫情。     

Role of neutrophil elastase and myeloperoxidase in asthma of rats

AIM:To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS:Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated andpurified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS:(1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P<0.01). Activities of MPO were significantly increased in bronchoalveolar lavage fluid (BALF) and lung tissue in asthma group compared with those in control group (P<0.05, P<0.01). (2) Levels of NE were significantly increased in BALF and PMN in asthma group compared with control group (P<0.01). (3) Number of PMN were significantly higher in BALF, bronchus and lung tissue in asthma group than those in control group (P<0.01). CONCLUSION:Levels of PMN counting and expression of MPO and NEare significantly increased in experimental asthma rats. PMN may be involved in inflammation in asthma via increasing the levels of NE and MPO.     

不同果袋类型对紫花杧的套袋效果

以主栽品种紫花杧为试材,探讨不同果袋类型的套袋效果.结果表明:套袋可大幅度降低采收果的果面流胶和雨水污染,极显著地降低果面虫伤率.套袋可改变采收时的果面颜色,但不同果袋类型间存在差异.后熟完成时,果面均为品种固有颜色,不因果袋种类而改变.套袋能促进果实后熟转黄和果色均匀,能显著降低炭疽病的病果率,但不同果袋对蒂腐病的防效存在较大差异.套袋能提高可溶性固形物含量和减轻果实失重.供试品种用外黄内黑复合纸袋套袋效果最为理想.     

Etoposide induces apoptosis via mitochondrial signaling pathway with cytochrome c release in Jurkat leukemia cells

AIM:To investigate the molecular mechanisms of apoptosis and to elucidate the apoptosis signaling pathway triggered by etoposide in Jurkat human leukemia cells. METHODS:Apoptosis was detected using annexin V-FITC and propidium iodide (PI) staining, respectively, and annexin V-FITC positive cells and hypodiploid cells were analyzed by flow cytometry. Mitochondrial membrane potential (△Ψm) was detected using 3, 3-dihexyloxycarbocyanine iodide ［DiOC6(3)］ staining and △Ψm low cells were analyzed by flow cytometry. Preparation of cytosolic extracts and isolation of mitochondria were completed by centrifugation. Western blotting analysis was used to evaluate the level of cytochrome c, caspase-3, and poly (ADP-ribose) polymerase (PARP) expression. RESULTS:Etoposide induced apoptosis showing phosphatidylserine externalization and DNA fragmentation in a time-dependent manner and the apoptosis could be inhibited by a broad caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (zVAD.fmk). Collapse of △Ψm induced by etoposide preceded DNA fragmentation and phosphatidylserine externalization. In contrast, it was not blocked by zVAD.fmk. Etoposide caused cytochrome c release from mitochondria into cytosol, subsequent activation of caspase-3 (32 kD) presented with an intermediate (20 kD) and its active product (17 kD), and cleavage of full-length PARP (116 kD) into the so-called apoptotic 85 kD fragment. CONCLUSION:Etoposide-induced Jurkat cell apoptosis is initiated through mitochondria signaling pathway with cytochrome c release into cytoplasm and caspase is the ultimate executioner of cell apoptosis.